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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
23/03/2016 |
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Data da última atualização: |
23/03/2016 |
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Autoria: |
ALCÂNTARA, B. K. de; ALFIERI, A. A.; HEADLEY, S. A.; RODRIGUES, W. B.; OTONEL, R. A. A.; LUNARDI, M.; ALFIERI, A. F. |
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Afiliação: |
BRÍGIDA K. DE ALCÂNTARA, DMVP/UEL; AMAURI A. ALFIERI, DMVP/UEL; SELWYN A. HEADLEY, DMVP/UEL; WAGNER B. RODRIGUES, DMVP/UEL; RODRIGO A. A. OTONEL, DMVP/UEL; MICHELE LUNARDI, UNIC; ALICE F. ALFIERI, DMVP/UEL. |
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Título: |
Molecular characterization of bovine Deltapapillomavirus (BPV1, 2, and 13) DNA in equine sarcoids. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro v. 35, n. 5, p. 431-436, maio 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Sarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreover, these findings represent the first description of three types of BPV diagnosed in the same horse, as well as the first confirmation of BPV1 and 2 in horses from Brazil. MenosSarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreove... Mostrar Tudo |
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Palavras-Chave: |
Bovine Deltapapillomavirus; Deltapapillomavirus bovino; Equine sarcoids; Horse; Papillomavirus; Sarcoide equino; Tumor de pele. |
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Thesagro: |
DNA. |
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Thesaurus Nal: |
Papillomaviridae; Skin neoplasms. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141614/1/Molecular-characterization.pdf
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Marc: |
LEADER 02588naa a2200313 a 4500
001 2041730
005 2016-03-23
008 2015 bl uuuu u00u1 u #d
100 1 $aALCÂNTARA, B. K. de
245 $aMolecular characterization of bovine Deltapapillomavirus (BPV1, 2, and 13) DNA in equine sarcoids.
260 $c2015
520 $aSarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreover, these findings represent the first description of three types of BPV diagnosed in the same horse, as well as the first confirmation of BPV1 and 2 in horses from Brazil.
650 $aPapillomaviridae
650 $aSkin neoplasms
650 $aDNA
653 $aBovine Deltapapillomavirus
653 $aDeltapapillomavirus bovino
653 $aEquine sarcoids
653 $aHorse
653 $aPapillomavirus
653 $aSarcoide equino
653 $aTumor de pele
700 1 $aALFIERI, A. A.
700 1 $aHEADLEY, S. A.
700 1 $aRODRIGUES, W. B.
700 1 $aOTONEL, R. A. A.
700 1 $aLUNARDI, M.
700 1 $aALFIERI, A. F.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 35, n. 5, p. 431-436, maio 2015.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 32 | |
| 5. |  | ANACHE, N. A.; SILVA, K. C.; RODRIGUES, W. B.; JARA, J. P.; CARDOSO, C. J. T.; NOGUEIRA, E. Estrus identification methods as an alternative to optimize IATF results. Animal Reprodroduction, v. 15, n. 3, p. 380, Jul./Sept. 2018. Abstract A065. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 6. | | PFEIFER, L. F. M.; RODRIGUES, W. B.; SILVA, K. C. da; ANACHE, N. A.; CASTRO, N. A.; CASTILHO, E. M.; NOGUEIRA, E. Different protocols using PGF2a as ovulation inducer in nelore cows subjected to estradiol-progesterone timed AI based protocols. Theriogenology, v. 120, p. 56-60, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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| 7. | | NOGUEIRA, E.; RODRIGUES, W. B.; SANCHES SILVA, C.; COSTA E SILVA, E. V.; POTIENS, J. R.; SILVA, J. C. B.; SUTOVSKY, P. Identification of seminal parameters predictive of conception rates in Angus and Nelore bulls used in TAI. Advances in Animal Biosciences, v. 9, part 2, p. 324, may 2018. Proceedings of the international Bull Fertility Conference, 2018, Westport.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 8. | | NOGUEIRA, E.; ABREU, U. G. P. de; OLIVEIRA, L. O. F. de; SILVA, J. C. B.; ITAVO, L. C. V.; DIAS, A. M.; RODRIGUES, W. B. Intensificación sustentable de sistemas de cría: técnicas de IATF y destete precoz. In: CONGRESSO INTERNACIONAL DE TRANSFERENCIA DE TECNOLOGÍA AGROPECUARIA, 28., 2015, Asunción, Paraguay. Cría y mejoramiento genético: anais. Asunción, Paraguay: CEA, 2015. p. 151-161. Na publicação: Juliana Correa Borges.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 9. | | ALCÂNTARA, B. K. de; ALFIERI, A. A.; HEADLEY, S. A.; RODRIGUES, W. B.; OTONEL, R. A. A.; LUNARDI, M.; ALFIERI, A. F. Molecular characterization of bovine Deltapapillomavirus (BPV1, 2, and 13) DNA in equine sarcoids. Pesquisa Veterinária Brasileira, Rio de Janeiro v. 35, n. 5, p. 431-436, maio 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 11. | | OLIVEIRA, L. O. F. de; NOGUEIRA, E.; GRI, A. L. da S.; SILVA, J. C. B.; RODRIGUES, W. B.; ABREU, U. G. P. de. Simulations of economic results with the use of FTAI in cow-calf farm in the Pantanal. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 52., 2015, Belo Horizonte. Zootecnia: otimizando recursos e potencialidades: anais. Brasília, DF: Sociedade Brasileira de Zootecnia, 2015. p. 1-3. Na publicação: Juliana Corrêa Borges.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 12. | | RODRIGUES, W. B.; SILVA, A. S.; SILVA, J. C. B.; ANACHE, N. A.; SILVA, K. C. da; CARDOSO, C. J. T.; GARCIA, W. R.; SUTOVSKY, P.; NOGUEIRA, E. Timed artificial insemination plus heat II: gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone/estradiol-based protocol. Animal, v. 13, n. 10, p. 2313-2318, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pantanal. |
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| 13. | | NOGUEIRA, E.; MINGOTI, G. ZOCCAL; NICACIO, A. C.; SILVA, J. C. B.; FERNANDES, C. A. C.; RODRIGUES, W. B.; MARQUES JÚNIOR, H. R. Utilização da ultrassonografia na reprodução de fêmeas bovinas. In: NOGUEIRA, E.; SILVA, J. C. B.; NICACIO, A. C.; MINGOTI, G. Z. (Ed.). Ultrassonografia na reprodução e avaliação de carcaças em bovinos. Brasília, DF: Embrapa, 2017. p. 37-93.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pantanal. |
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| 14. | | NOGUEIRA, E.; MINGOTI, G. ZOCCAL; NICACIO, A. C.; SILVA, J. C. B.; FERNANDES, C. A. C.; RODRIGUES, W. B.; MARQUES JÚNIOR, H. R. Utilização da ultrassonografia na reprodução de fêmeas bovinas. In: NOGUEIRA, E.; SILVA, J. C. B.; NICACIO, A. C.; MINGOTI, G. Z. (Ed.). Ultrassonografia na reprodução e avaliação de carcaças em bovinos. Brasília, DF: Embrapa, 2021. Cap. 2. E-book.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pantanal. |
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| 15. | | SILVA, M. V. G. B.; MARTINS, M. F.; ARBEX, W. A.; PAIVA, L. DE C.; CEMBRANELLI, M. DE A. R.; RODRIGUES, W. B. R. Usando a ciência como ferramenta para a escolha de reprodutores. Girolando, v. 15, n. 87, p. 18-21, 2012.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | RODRIGUES, W. B.; JARA, J. do P.; SILVA, J. C. B.; OLIVEIRA, L. O. F. de; ABREU, U. G. P. de; SILVA, K. C. da; ANACHE, N. A.; OLIVEIRA, A. B. de; CARDOSO, C. J. T.; NOGUEIRA, E. Acão da Prostaglandina F2a como indutor de ovulação em vacas de corte submetidas a protocolos de IATF. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 22., 2017, Santos, SP. Anais... Belo Horizonte: CBRA, 2017. p. 374. Na publicação: Juliana Correa Borges.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 17. | | RODRIGUES, W. B.; JARA, J. do P.; SILVA, J. C. B.; OLIVEIRA, L. O. F. de; ABREU, U. G. P. de; ANACHE, N. A.; SILVA, K. C. da; BEZERRA, A. de O.; CARDOSO, C. J. T.; NOGUEIRA, E. Efficiency of mating, artificial insemination or resynchronisation at different times after first timed artificial insemination in postpartum Nellore cows to produce crossbred calves. Animal Production Science, v. 59, n. 2, p. 225-231, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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| 18. | | RODRIGUES, W. B.; JARA, J. do P.; SILVA, J. C. B.; OLIVEIRA, L. O. F. de; ABREU, U. G. P. de; BATISTA, D. S. do N.; ANACHE, N. A.; SILVA, K. C.; BEZERRA, A. O.; NOGUEIRA, E. Efficiency of post FTAI manegement in lacting beef cows. Animal Reproduction, v. 13, n. 3, p. 452, Jul./Sept. 2016. Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain,...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 19. | | CARDOSO, C. J. T.; LIMA, A. C. B.; PEREIRA, D. M.; SILVA, G. C.; SILVA, K. C.; ANACHE, N. A.; RODRIGUES, W. B.; MARQUES JÚNIOR, H. R.; MELO-STERZA, F. A.; NOGUEIRA, E. Effects of narasin on the pregnancy rate of Nellore cows maintained under grazing and performance of calves - preliminary results. Animal Reproduction, v. 15, n. 3, p. 362, jul./sept. 2018. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 20. | | NOGUEIRA, E.; SILVA, J. C. B.; SILVA, M. R.; SILVA, A. S.; RODRIGUES, W. B.; BEZERRA, A. O.; JARA, J. do P.; SILVA, K. C. da; ANACHE, N. A. IATF + CIO: estratégia prática de avaliação de cio e aumento de prenhez. Corumbá: Embrapa Pantanal, 2016. 8 p. (Embrapa Pantanal. Circular Técnica, 113).| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 32 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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